A Map of the Interactome Network of the Metazoan C. elegans
Harvard University · University of Namur · +7 more institutions
Abstract
To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains approximately 5500 interactions. Topological and biological features of this interactome network, as well…
Citation impact
- FWCI
- 72.19
- Percentile
- 100%
- References
- 27
Authors
48- SLSiming LiCorresponding
Harvard University, University of Namur, Baylor College of Medicine, Institut de Génétique Moléculaire de Montpellier, Dana-Farber Cancer Institute
- CMChristopher M. Armstrong
Harvard University, University of Namur, Baylor College of Medicine, Institut de Génétique Moléculaire de Montpellier, Dana-Farber Cancer Institute
- NBNicolas Bertin
Harvard University, University of Namur, Baylor College of Medicine, Institut de Génétique Moléculaire de Montpellier, Dana-Farber Cancer Institute
- HMHui Ming Ge
Harvard University, University of Namur, Baylor College of Medicine, Institut de Génétique Moléculaire de Montpellier, Dana-Farber Cancer Institute
- SMStuart Milstein
Harvard University, University of Namur, Baylor College of Medicine, Institut de Génétique Moléculaire de Montpellier, Dana-Farber Cancer Institute
Topics & keywords
- Interactome
- In silico
- Phenome
- Computational biology
- Biology
- Biological network
- Multicellular organism
- Interaction network