Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)
California NanoSystems Institute · University of California, Los Angeles · +2 more institutions
Abstract
Super-resolution optical microscopy is a rapidly evolving area of fluorescence microscopy with a tremendous potential for impacting many fields of science. Several super-resolution methods have been developed over the last decade, all capable of overcoming the fundamental diffraction limit of light. We present here an approach for obtaining subdiffraction limit optical resolution in all three dimensions. This method relies on higher-order statistical analysis of temporal fluctuations (caused by fluorescence blinking/intermittency) recorded in a sequence of images (movie). We demonstrate a 5-fold improvement in spatial resolution by using a conventional wide-field microscope. This resolution enhancement is…
Citation impact
- FWCI
- 24.27
- Percentile
- 100%
- References
- 32
Authors
5- TDThomas DertingerCorresponding
California NanoSystems Institute, University of California, Los Angeles
- RARyan A. Colyer
University of California, San Francisco
- GIGopal Iyer
University of California, San Francisco
- SWShimon Weiss
California NanoSystems Institute, University of California, Los Angeles
- JEJörg Enderlein
University of Göttingen
Topics & keywords
- Resolution (logic)
- Microscopy
- Optics
- Image resolution
- Microscope
- Intermittency
- Superresolution
- Super-resolution microscopy