Ribosome Profiling Reveals Pervasive Translation Outside of Annotated Protein-Coding Genes
Carnegie Institution for Science · Department of Embryology · +5 more institutions
Abstract
Ribosome profiling suggests that ribosomes occupy many regions of the transcriptome thought to be noncoding, including 5' UTRs and long noncoding RNAs (lncRNAs). Apparent ribosome footprints outside of protein-coding regions raise the possibility of artifacts unrelated to translation, particularly when they occupy multiple, overlapping open reading frames (ORFs). Here, we show hallmarks of translation in these footprints: copurification with the large ribosomal subunit, response to drugs targeting elongation, trinucleotide periodicity, and initiation at early AUGs. We develop a metric for distinguishing between 80S footprints and nonribosomal sources using footprint size distributions, which validates the vast…
Citation impact
- FWCI
- 27.46
- Percentile
- 100%
- References
- 53
Authors
8- NTNicholas T. IngoliaCorresponding
Carnegie Institution for Science, Department of Embryology
- GAGloria A. Brar
University of California, San Francisco, QB3, Howard Hughes Medical Institute
- NSNoam Stern‐Ginossar
University of California, San Francisco, Howard Hughes Medical Institute, QB3
- MSM. Scott Harris
Johns Hopkins University, Carnegie Institution for Science, Department of Embryology
- GJGaëlle J.S. Talhouarne
Department of Embryology, Johns Hopkins University, Carnegie Institution for Science
Topics & keywords
- Ribosome profiling
- Biology
- Open reading frame
- Transcriptome
- Computational biology
- Ribosome
- ORFS
- Translation (biology)
- Quality Education