Plant host habitat and root exudates shape soil bacterial community structure
Centre National de la Recherche Scientifique · Commissariat à l'Énergie Atomique et aux Énergies Alternatives · +4 more institutions
Abstract
The rhizosphere is active and dynamic in which newly generated carbon, derived from root exudates, and ancient carbon, in soil organic matter (SOM), are available for microbial growth. Stable isotope probing (SIP) was used to determine bacterial communities assimilating each carbon source in the rhizosphere of four plant species. Wheat, maize, rape and barrel clover (Medicago truncatula) were grown separately in the same soil under (13)CO(2) (99% of atom (13)C) and DNA extracted from rhizosphere soil was fractionated by isopycnic centrifugation. Bacteria-assimilating root exudates were characterized by denaturing gradient gel electrophoresis (DGGE) analysis of (13)C-DNA and root DNA, whereas those assimilating…
Citation impact
- FWCI
- 20.87
- Percentile
- 100%
- References
- 53
Authors
8- FEFeth el Zahar HaicharCorresponding
Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Ecologie Microbienne Lyon
- CMC. Marol
Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, CEA Cadarache, Ecologie Microbienne Lyon
- OBOdile Berge
Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Ecologie Microbienne Lyon
- JIJ. Ignacio Rangel‐Castro
University of Aberdeen
- JIJames I. Prosser
University of Aberdeen
Topics & keywords
- Rhizosphere
- Biology
- Temperature gradient gel electrophoresis
- Botany
- Bulk soil
- Stable-isotope probing
- Bacteria
- Actinobacteria
- Life in Land