articleCancer ResearchDec 15, 2012Closed access

The Noncoding RNA MALAT1 Is a Critical Regulator of the Metastasis Phenotype of Lung Cancer Cells

TGTony GutschnerMHMonika HämmerleMFMoritz F. EissmannJHJeff HsuYKYoungsoo Kim

German Cancer Research Center · Heidelberg University · +4 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

The long noncoding RNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1), also known as MALAT-1 or NEAT2 (nuclear-enriched abundant transcript 2), is a highly conserved nuclear noncoding RNA (ncRNA) and a predictive marker for metastasis development in lung cancer. To uncover its functional importance, we developed a MALAT1 knockout model in human lung tumor cells by genomically integrating RNA destabilizing elements using zinc finger nucleases. The achieved 1,000-fold MALAT1 silencing provides a unique loss-of-function model. Proposed mechanisms of action include regulation of splicing or gene expression. In lung cancer, MALAT1 does not alter alternative splicing but actively regulates gene…

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Topics & keywords

Topics
Keywords
  • MALAT1
  • Metastasis
  • Cancer research
  • Long non-coding RNA
  • Lung cancer
  • Gene silencing
  • Biology
  • Adenocarcinoma
UN Sustainable Development Goals
  • Good health and well-being
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