Circular dichroism and conformational polymorphism of DNA
Czech Academy of Sciences, Institute of Biophysics · Czech Academy of Sciences
Abstract
Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast and simple method can be used at low- as well as high-DNA concentrations and with short- as well as long-DNA molecules. The samples can easily be titrated with various agents to cause conformational isomerizations of DNA. The course of detected CD spectral changes makes possible to distinguish between gradual changes…
Citation impact
- FWCI
- 21.35
- Percentile
- 100%
- References
- 98
Authors
4- JKJaroslav KyprCorresponding
Czech Academy of Sciences, Institute of Biophysics, Czech Academy of Sciences
- IKIva Kejnovská
Czech Academy of Sciences, Czech Academy of Sciences, Institute of Biophysics
- DRDaniel Renčiuk
Czech Academy of Sciences, Institute of Biophysics, Czech Academy of Sciences
- MVMichaela Vorlı́čková
Czech Academy of Sciences, Czech Academy of Sciences, Institute of Biophysics
Topics & keywords
- Circular dichroism
- DNA
- Conformational isomerism
- Guanine
- Cytosine
- Spectroscopy
- Conformational change
- Molecule