articleThe Plant CellMar 1, 2007BRONZE OA

Calcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH Oxidase

MKMichie KobayashiIOIkuko OhuraKKKazuhito KawakitaNYNaohiko YokotaMFMasayuki Fujiwara

Nagoya University · Nara Institute of Science and Technology

PubMed
Indexed incrossrefpubmed

Abstract

Reactive oxygen species (ROS) are implicated in plant innate immunity. NADPH oxidase (RBOH; for Respiratory Burst Oxidase Homolog) plays a central role in the oxidative burst, and EF-hand motifs in the N terminus of this protein suggest possible regulation by Ca(2+). However, regulatory mechanisms are largely unknown. We identified Ser-82 and Ser-97 in the N terminus of potato (Solanum tuberosum) St RBOHB as potential phosphorylation sites. An anti-phosphopeptide antibody (pSer82) indicated that Ser-82 was phosphorylated by pathogen signals in planta. We cloned two potato calcium-dependent protein kinases, St CDPK4 and St CDPK5, and mass spectrometry analyses showed that these CDPKs phosphorylated only Ser-82…

Citation impact

665
total citations
FWCI
40.03
Percentile
100%
References
82
Citations per year

Authors

8

Topics & keywords

Topics
Keywords
  • NADPH oxidase
  • Biology
  • Reactive oxygen species
  • Oxygen
  • Calcium
  • Kinase
  • Biochemistry
  • Cell biology
UN Sustainable Development Goals
  • Life in Land
No related works found for this paper.

Funding