A physical, genetic and functional sequence assembly of the barley genome

Gundlach, Heidrun; Pfeifer, Matthias; Nussbaumer, Thomas; Mayer, Klaus; Spannagl, M.; Martis, Mihaela; Waugh, Robbie; Russell, Joanne; Liu, Hui; Druka, Arnis; Brown, John W. S.; Waugh, Robbie; Morris, Jenny; Marshall, David; Hedley, Pete E.; Bayer, Micha; Langridge, Peter; Langridge, Peter; Shi, Bu-Jun; Langridge, Peter; Madishetty, Kavitha; Wannamaker, Steve; Resnik, Josh; Bhat, Prasanna R.; Moscou, Matthew; Close, Timothy J.; Wanamaker, Steve; Close, Timothy J.; Wise, Roger P.; Wise, Roger P.; Wise, Roger P.; Graner, Andreas; Himmelbach, Axel; Schulte, Daniela; Poursarebani, Naser; Ariyadasa, Ruvini; Stein, Nils; Zhou, Ruonan; Steuernagel, Burkhard; Scholz, Uwe; Mascher, Martin; Schmutzer, Thomas; Graner, Andreas; Matsumoto, Takashi; Matsumoto, Takashi; Tanaka, Tsuyoshi; Sato, Kazuhiro; Sato, Kazuhiro; Sato, Kazuhiro; Schulman, Alan H.; Schulman, Alan H.; Tanskanen, Jaakko; Moisy, Cédric; Muehlbauer, Gary J.; Muehlbauer, Gary J.; Muehlbauer, Gary J.; Frenkel, Zeev; Korol, A.; Bergès, Hélène; Platzer, Matthias; Groth, Marco; Felder, Marius; Platzer, Matthias; Taudien, Stefan; Taudien, Stefan; Duma, Denisa; Cordero, Francesa; Beccuti, Marco; Ciardo, Gianfranco; Alpert, Matthew; Ma, Yaqin; Lonardi, Stefano; Zuccolo, Andrea; Morgante, Michele; Cattonaro, Federica; Scalabrin, Simone; Vendramin, Vera; Morgante, Michele; Vendramin, Vera; Radović, Slobodanka; Wing, Rod A.; Poland, Jesse; Ayling, Sarah; Swarbreck, David; Sampath, Dharanya; Cáccamo, Mario; Febrer, Melanie; Brown, John W. S.; Fincher, Geoffrey B.; Cordero, Francesa

doi:10.1038/nature11543

articleNatureOct 16, 2012HYBRID OA

A physical, genetic and functional sequence assembly of the barley genome

HGHeidrun Gundlach MPMatthias Pfeifer TNThomas Nussbaumer KMKlaus Mayer MSM. Spannagl

Helmholtz Zentrum München · James Hutton Institute · +30 more institutions

PubMed

Indexed incrossrefpubmed

Abstract

Barley (Hordeum vulgare L.) is among the world’s earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 ‘high-confidence’ genes with homology support from other plant genomes. Abundant alternative splicing,…

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Topics & keywords

Topics

Keywords

Sequence (biology)
Genome
Biology
Computational biology
Whole genome sequencing
Sequence assembly
Genetics
Gene

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