Genome-wide RNAi screen of Ca 2+ influx identifies genes that regulate Ca 2+ release-activated Ca 2+ channel activity
University of California, Irvine · Columbia University · +3 more institutions
Abstract
Recent studies by our group and others demonstrated a required and conserved role of Stim in store-operated Ca(2+) influx and Ca(2+) release-activated Ca(2+) (CRAC) channel activity. By using an unbiased genome-wide RNA interference screen in Drosophila S2 cells, we now identify 75 hits that strongly inhibited Ca(2+) influx upon store emptying by thapsigargin. Among these hits are 11 predicted transmembrane proteins, including Stim, and one, olf186-F, that upon RNA interference-mediated knockdown exhibited a profound reduction of thapsigargin-evoked Ca(2+) entry and CRAC current, and upon overexpression a 3-fold augmentation of CRAC current. CRAC currents were further increased to 8-fold higher than control…
Citation impact
- FWCI
- 23.73
- Percentile
- 100%
- References
- 24
Authors
9- SLShenyuan L. ZhangCorresponding
University of California, Irvine
- AVAndriy V. Yeromin
University of California, Irvine
- XHXiang H.-F. Zhang
Columbia University
- YYYing Yu
Chinese Academy of Sciences, University of California, Irvine, Institute of Atmospheric Physics
- OSOlga Safrina
University of California, Irvine
Topics & keywords
- Thapsigargin
- SERCA
- ORAI1
- RNA interference
- STIM1
- Endoplasmic reticulum
- Cell biology
- Chemistry