Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes
Max Planck Society · Heidelberg University · +3 more institutions
Abstract
Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sorting of membrane into the different populations of intraluminal vesicles are unknown. Here, we find that cargo is segregated into distinct subdomains on the endosomal membrane and that the transfer of exosome-associated domains into the lumen of the endosome did not depend on the function of the ESCRT (endosomal sorting complex required for transport) machinery, but required the sphingolipid ceramide. Purified exosomes were enriched in ceramide, and the release of exosomes was reduced after the inhibition of neutral…
Citation impact
- FWCI
- 30.04
- Percentile
- 100%
- References
- 24
Authors
9- KTKatarina TrajkovićCorresponding
Max Planck Society, Heidelberg University, Max Planck Institute for Biophysical Chemistry, University of Göttingen, Technische Universität Dresden
- CHChieh HsuCorresponding
Max Planck Society, Heidelberg University, Max Planck Institute for Biophysical Chemistry, University of Göttingen, Technische Universität Dresden
- SCSalvatore Chiantia
Max Planck Society, Heidelberg University, Max Planck Institute for Biophysical Chemistry, University of Göttingen, Technische Universität Dresden
- LRLawrence Rajendran
Max Planck Society, Heidelberg University, Max Planck Institute for Biophysical Chemistry, University of Göttingen, Technische Universität Dresden
- DWDirk Wenzel
Max Planck Society, Heidelberg University, Max Planck Institute for Biophysical Chemistry, University of Göttingen, Technische Universität Dresden
Topics & keywords
- Endosome
- ESCRT
- Cell biology
- Microvesicles
- Exosome
- Ceramide
- Vesicle
- Exocytosis
- Life below water