Molecular analysis of the bacterial microbiota in the human stomach

Bik, Elisabeth M.; Eckburg, Paul B.; Gill, Steven R.; Nelson, Karen E.; Purdom, Elizabeth; François, Fritz; Perez-Perez, Guillermo; Blaser, Martin J.; Relman, David A.

doi:10.1073/pnas.0506655103

articleProceedings of the National Academy of SciencesJan 4, 2006GREEN OA

Molecular analysis of the bacterial microbiota in the human stomach

EMElisabeth M. Bik PBPaul B. Eckburg SRSteven R. GillKEKaren E. NelsonEPElizabeth Purdom

Stanford University · VA Palo Alto Health Care System · +2 more institutions

PubMed

Indexed incrossrefpubmed

Abstract

The microbiota of the human stomach and the influence of Helicobacter pylori colonization on its composition remain largely unknown. We characterized bacterial diversity within the human gastric mucosa by using a small subunit 16S rDNA clone library approach and analyzed 1,833 sequences generated by broad-range bacterial PCR from 23 gastric endoscopic biopsy samples. A diverse community of 128 phylotypes was identified, featuring diversity at this site greater than previously described. The majority of sequences were assigned to the Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Fusobacteria phyla. Ten percent of the phylotypes were previously uncharacterized, including a Deinococcus-related…

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Authors

9

EM
Elisabeth M. Bik
Stanford University
PB
Paul B. Eckburg
VA Palo Alto Health Care System, New York University, Stanford University
SR
Steven R. Gill
Sequoia (United States), New York University, Stanford University
KE
Karen E. Nelson
Sequoia (United States), New York University, Stanford University
EP
Elizabeth Purdom
New York University, Stanford University

Topics & keywords

Topics

Keywords

Fusobacteria
Biology
Phylotype
Bacteroidetes
Proteobacteria
Firmicutes
Actinobacteria
Helicobacter pylori

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