articleRNAAug 15, 2003BRONZE OA

siRNA function in RNAi: A chemical modification analysis

YCYa‐Lin ChiuTMTariq M. Rana

University of Massachusetts Chan Medical School

PubMed
Indexed incrossrefpubmed

Abstract

Various chemical modifications were created in short-interfering RNAs (siRNAs) to determine the biochemical properties required for RNA interference (RNAi). Remarkably, modifications at the 2'-position of pentose sugars in siRNAs showed the 2'-OHs were not required for RNAi, indicating that RNAi machinery does not require the 2'-OH for recognition of siRNAs and catalytic ribonuclease activity of RNA-induced silencing complexes (RISCs) does not involve the 2'-OH of guide antisense RNA. In addition, 2' modifications predicted to stabilize siRNA increased the persistence of RNAi as compared with wild-type siRNAs. RNAi was also induced with chemical modifications that stabilized interactions between A-U base…

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Authors

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Topics & keywords

Topics
Keywords
  • RNA interference
  • Small interfering RNA
  • Biology
  • Trans-acting siRNA
  • RNA silencing
  • RNA
  • Gene silencing
  • Sense (electronics)
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