A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial–mesenchymal transition
Hospital Del Mar · Municipal Institute for Medical Research · +4 more institutions
Abstract
Expression of Snail1 in epithelial cells triggers an epithelial-mesenchymal transition (EMT). Here, we demonstrate that the synthesis of Zeb2, a transcriptional repressor of E-cadherin, is up-regulated after Snail1-induced EMT. Snail1 does not affect the synthesis of Zeb2 mRNA, but prevents the processing of a large intron located in its 5'-untranslated region (UTR). This intron contains an internal ribosome entry site (IRES) necessary for the expression of Zeb2. Maintenance of 5'-UTR Zeb2 intron is dependent on the expression of a natural antisense transcript (NAT) that overlaps the 5' splice site in the intron. Ectopic overexpression of this NAT in epithelial cells prevents splicing of the Zeb2 5'-UTR,…
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Authors
8- MBManuel BeltránCorresponding
Hospital Del Mar, Municipal Institute for Medical Research
- IPIsabel Puig
Institut Municipal d'Assistència Sanitària, Hospital Del Mar, Municipal Institute for Medical Research, Hospital del Mar Research Institute
- CPCristina Peña
Hospital Universitario Puerta de Hierro Majadahonda
- JMJosé Miguel García
Hospital Universitario Puerta de Hierro Majadahonda
- ABAna Belén Cid Álvarez
Hospital Del Mar, Municipal Institute for Medical Research
Topics & keywords
- Biology
- Intron
- Untranslated region
- RNA splicing
- Epithelial–mesenchymal transition
- Internal ribosome entry site
- Three prime untranslated region
- Molecular biology
- Life in Land