Sequence signatures and mRNA concentration can explain two‐thirds of protein abundance variation in a human cell line
The University of Texas at Austin · The University of Texas at San Antonio Health Science Center · +5 more institutions
Abstract
Transcription, mRNA decay, translation and protein degradation are essential processes during eukaryotic gene expression, but their relative global contributions to steady-state protein concentrations in multi-cellular eukaryotes are largely unknown. Using measurements of absolute protein and mRNA abundances in cellular lysate from the human Daoy medulloblastoma cell line, we quantitatively evaluate the impact of mRNA concentration and sequence features implicated in translation and protein degradation on protein expression. Sequence features related to translation and protein degradation have an impact similar to that of mRNA abundance, and their combined contribution explains two-thirds of protein abundance…
Citation impact
- FWCI
- 18.37
- Percentile
- 100%
- References
- 72
Authors
10- CVChristine VogelCorresponding
The University of Texas at Austin
- RDRaquel de Sousa Abreu
The University of Texas at San Antonio Health Science Center, Children's Cancer Center
- DKDaijin Ko
The University of Texas at San Antonio
- SLShu‐Yun Le
Frederick National Laboratory for Cancer Research, National Cancer Institute, Center for Cancer Research
- BABruce A. Shapiro
Frederick National Laboratory for Cancer Research, National Cancer Institute, Center for Cancer Research
Topics & keywords
- Biology
- Messenger RNA
- Untranslated region
- Coding region
- Gene expression
- Protein biosynthesis
- Gene
- Five prime untranslated region