Mobilization of hematopoietic progenitor cells in healthy volunteers by AMD3100, a CXCR4 antagonist
University of Washington · Walther Cancer Foundation · +1 more institution
Abstract
Stromal cell-derived factor 1 (SDF1/CXCL12) and its cognate receptor, CXCR4, play key regulatory roles in CD34+ cell trafficking. We investigated whether AMD3100, a selective CXCR4 antagonist, could mobilize hematopoietic progenitor cells from marrow to peripheral blood in healthy human volunteers. Initially, 10 persons each received a single dose of AMD3100 (80 microsubcutaneously), which induced rapid, generalized leukocytosis associated with an increase in peripheral blood CD34+ cells, representing pluripotent hematopoietic progenitors by in vitro colony-forming unit assays, from 3.8 +/- 0.5/microL to 20.7 +/- 3.5/microL at 6 hours. Subsequent dose-response studies showed a maximum increase in circulating…
Citation impact
- FWCI
- 13.92
- Percentile
- 100%
- References
- 29
Authors
11- WCW. Conrad LilesCorresponding
University of Washington, Walther Cancer Foundation, Indiana University – Purdue University Indianapolis
- HEHal E. Broxmeyer
University of Washington, Walther Cancer Foundation, Indiana University – Purdue University Indianapolis
- ERElin Rodger
University of Washington, Walther Cancer Foundation, Indiana University – Purdue University Indianapolis
- BLBrent L. Wood
University of Washington, Walther Cancer Foundation, Indiana University – Purdue University Indianapolis
- KHKai Hübel
University of Washington, Walther Cancer Foundation, Indiana University – Purdue University Indianapolis
Topics & keywords
- CD34
- Progenitor cell
- Haematopoiesis
- CXCR4
- Plerixafor
- Stromal cell
- Immunology
- Stem cell