Radiation Response of Mammalian Cells Grown in Culture I. Repair of X-Ray Damage in Surviving Chinese Hamster Cells

The generally observed threshold type of response of the lethal effect of x rays on cultured mammaliam cells means that damage must be accumulated before a lethal response is prcduced. Surviving cells are, therefore, damaged cells. A study was undertaken, using two-cell lines of the Chinese hamster propagated in vitro, to determine whether or not sublethal damage is heritsble, as might be expected if lethal damage is primarily genic. Using 55-kv x rays, glass-attached cells, and colony formation as a criterion of survival, it was found that the cell lines studied displayed mean lethal doses close to those found with other mammalian cell lines (100 to 150 rads). In contrast to other mamlian cell lines, the…