A Cas9–guide RNA complex preorganized for target DNA recognition
University of California, Berkeley · Howard Hughes Medical Institute · +4 more institutions
Abstract
Bacterial adaptive immunity uses CRISPR (clustered regularly interspaced short palindromic repeats)-associated (Cas) proteins together with CRISPR transcripts for foreign DNA degradation. In type II CRISPR-Cas systems, activation of Cas9 endonuclease for DNA recognition upon guide RNA binding occurs by an unknown mechanism. Crystal structures of Cas9 bound to single-guide RNA reveal a conformation distinct from both the apo and DNA-bound states, in which the 10-nucleotide RNA "seed" sequence required for initial DNA interrogation is preordered in an A-form conformation. This segment of the guide RNA is essential for Cas9 to form a DNA recognition-competent structure that is poised to engage double-stranded DNA…
Citation impact
- FWCI
- 29.36
- Percentile
- 100%
- References
- 43
Authors
5- FJFuguo Jiang
University of California, Berkeley
- KZKaihong Zhou
Howard Hughes Medical Institute, University of California, Berkeley
- LMLinlin Ma
Howard Hughes Medical Institute, University of California, Berkeley
- SGSaskia Gressel
Max Planck Institute for Biophysical Chemistry
- JAJennifer A. DoudnaCorresponding
QB3, Howard Hughes Medical Institute, Lawrence Berkeley National Laboratory, Innovative Genomics Institute, University of California, Berkeley
Topics & keywords
- Cas9
- RNA
- CRISPR
- Guide RNA
- Biology
- DNA
- Computational biology
- Nuclease
- Life in Land