Discordant Protein and mRNA Expression in Lung Adenocarcinomas

Chen, Guoan; Gharib, Tarek G.; Huang, Chiang-Ching; Taylor, Jeremy M. G.; Misek, David E.; Kardia, Sharon L. R.; Giordano, Thomas J.; Iannettoni, Mark D.; Orringer, Mark B.; Hanash, Samir; Beer, David G.

doi:10.1074/mcp.m200008-mcp200

articleMolecular & Cellular ProteomicsApr 1, 2002HYBRID OA

Discordant Protein and mRNA Expression in Lung Adenocarcinomas

GCGuoan Chen TGTarek G. Gharib CHChiang-Ching Huang JMJeremy M. G. Taylor DEDavid E. Misek

University of Michigan–Ann Arbor

PubMed

Indexed incrossrefdoajpubmed

Abstract

The relationship between gene expression measured at the mRNA level and the corresponding protein level is not well characterized in human cancer. In this study, we compared mRNA and protein expression for a cohort of genes in the same lung adenocarcinomas. The abundance of 165 protein spots representing 98 individual genes was analyzed in 76 lung adenocarcinomas and nine non-neoplastic lung tissues using two-dimensional polyacrylamide gel electrophoresis. Specific polypeptides were identified using matrix-assisted laser desorption/ionization mass spectrometry. For the same 85 samples, mRNA levels were determined using oligonucleotide microarrays, allowing a comparative analysis of mRNA and protein expression…

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Authors

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Topics & keywords

Topics

Keywords

Messenger RNA
Molecular biology
Gene
Gene expression
Biology
DNA microarray
Genetics

UN Sustainable Development Goals

Good health and well-being

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Funding

NI
National Institutes of Health