The colorectal microRNAome
Howard Hughes Medical Institute · Johns Hopkins University · +7 more institutions
Abstract
MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of…
Citation impact
- FWCI
- 42.32
- Percentile
- 100%
- References
- 34
Authors
16- JMJordan M. Cummins
Howard Hughes Medical Institute, Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center
- YHYiping He
Howard Hughes Medical Institute, Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center
- RLRebecca Leary
Howard Hughes Medical Institute, Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center
- RPRay Pagliarini
Howard Hughes Medical Institute, Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center
- LALuis A. Díaz
Howard Hughes Medical Institute, Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center
Topics & keywords
- microRNA
- Biology
- Computational biology
- Dicer
- Human genome
- Genetics
- Multicellular organism
- MiRBase
- Good health and well-being