articlePLANT PHYSIOLOGYOct 23, 2009HYBRID OA

Uncovering Small RNA-Mediated Responses to Phosphate Deficiency in Arabidopsis by Deep Sequencing

National Chung Hsing University · University of Chicago · +6 more institutions

PubMed
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Abstract

Recent studies have demonstrated the important role of plant microRNAs (miRNAs) under nutrient deficiencies. In this study, deep sequencing of Arabidopsis (Arabidopsis thaliana) small RNAs was conducted to reveal miRNAs and other small RNAs that were differentially expressed in response to phosphate (Pi) deficiency. About 3.5 million sequence reads corresponding to 0.6 to 1.2 million unique sequence tags from each Pi-sufficient or Pi-deficient root or shoot sample were mapped to the Arabidopsis genome. We showed that upon Pi deprivation, the expression of miR156, miR399, miR778, miR827, and miR2111 was induced, whereas the expression of miR169, miR395, and miR398 was repressed. We found cross talk coordinated…

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Authors

8

Topics & keywords

Keywords
  • Arabidopsis
  • Biology
  • Small RNA
  • microRNA
  • RNA
  • Deep sequencing
  • Genetics
  • Small interfering RNA
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