A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield
International AIDS Vaccine Initiative · Scripps Research Institute · +10 more institutions
Abstract
The HIV envelope (Env) protein gp120 is protected from antibody recognition by a dense glycan shield. However, several of the recently identified PGT broadly neutralizing antibodies appear to interact directly with the HIV glycan coat. Crystal structures of antigen-binding fragments (Fabs) PGT 127 and 128 with Man(9) at 1.65 and 1.29 angstrom resolution, respectively, and glycan binding data delineate a specific high mannose-binding site. Fab PGT 128 complexed with a fully glycosylated gp120 outer domain at 3.25 angstroms reveals that the antibody penetrates the glycan shield and recognizes two conserved glycans as well as a short β-strand segment of the gp120 V3 loop, accounting for its high binding affinity…
Citation impact
- FWCI
- 28.37
- Percentile
- 100%
- References
- 75
Authors
31- RPRobert PejchalCorresponding
International AIDS Vaccine Initiative
- KJKatie J. Doores
Scripps Research Institute, International AIDS Vaccine Initiative, Ragon Institute of MGH, MIT and Harvard
- LMLaura M. Walker
Scripps Research Institute, International AIDS Vaccine Initiative
- RKReza Khayat
International AIDS Vaccine Initiative
- PHPo‐Ssu Huang
University of Washington
Topics & keywords
- Glycan
- Antibody
- Neutralization
- Immunoglobulin Fab Fragments
- Chemistry
- Binding site
- Neutralizing antibody
- Virology
- Good health and well-being