Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche
Albert Einstein College of Medicine · Icahn School of Medicine at Mount Sinai · +4 more institutions
Abstract
Hematopoietic stem cells (HSCs) reside in specialized bone marrow (BM) niches regulated by the sympathetic nervous system (SNS). Here, we have examined whether mononuclear phagocytes modulate the HSC niche. We defined three populations of BM mononuclear phagocytes that include Gr-1(hi) monocytes (MOs), Gr-1(lo) MOs, and macrophages (MΦ) based on differential expression of Gr-1, CD115, F4/80, and CD169. Using MO and MΦ conditional depletion models, we found that reductions in BM mononuclear phagocytes led to reduced BM CXCL12 levels, the selective down-regulation of HSC retention genes in Nestin(+) niche cells, and egress of HSCs/progenitors to the bloodstream. Furthermore, specific depletion of CD169(+) MΦ,…
Citation impact
- FWCI
- 35.82
- Percentile
- 100%
- References
- 48
Authors
13- ACAndrew Chow
Albert Einstein College of Medicine, Icahn School of Medicine at Mount Sinai
- DLDaniel Lucas
Albert Einstein College of Medicine, Icahn School of Medicine at Mount Sinai
- AHAndrés Hidalgo
Spanish National Centre for Cardiovascular Research, Thrombosis Research Institute, Icahn School of Medicine at Mount Sinai
- SMSimón Méndez‐Ferrer
Spanish National Centre for Cardiovascular Research, Thrombosis Research Institute, Icahn School of Medicine at Mount Sinai
- DHDaigo Hashimoto
Icahn School of Medicine at Mount Sinai
Topics & keywords
- Cell biology
- Progenitor cell
- Haematopoiesis
- Stem cell
- Biology
- Bone marrow
- Mesenchymal stem cell
- Immunology