G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis
Kyoto University · Osaka Prefecture University · +6 more institutions
Abstract
Covalent modification of histone tails is crucial for transcriptional regulation, mitotic chromosomal condensation, and heterochromatin formation. Histone H3 lysine 9 (H3-K9) methylation catalyzed by the Suv39h family proteins is essential for establishing the architecture of pericentric heterochromatin. We recently identified a mammalian histone methyltransferase (HMTase), G9a, which has strong HMTase activity towards H3-K9 in vitro. To investigate the in vivo functions of G9a, we generated G9a-deficient mice and embryonic stem (ES) cells. We found that H3-K9 methylation was drastically decreased in G9a-deficient embryos, which displayed severe growth retardation and early lethality. G9a-deficient ES cells…
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Authors
11- MTMakoto TachibanaCorresponding
Kyoto University
- KSKenji Sugimoto
Osaka Prefecture University, The University of Osaka
- MNMasami Nozaki
The University of Osaka
- JUJun Ueda
Kyoto University, Kyoto University of Education, The University of Osaka
- TOTsutomu Ohta
National Cancer Research Institute, The University of Osaka
Topics & keywords
- Biology
- EZH2
- Histone H3
- Euchromatin
- Histone methyltransferase
- Histone methylation
- Heterochromatin protein 1
- Methylation
- Good health and well-being