A macrophage colony-stimulating factor receptor–green fluorescent protein transgene is expressed throughout the mononuclear phagocyte system of the mouse
Albert Einstein College of Medicine · The University of Queensland · +4 more institutions
Abstract
The c-fms gene encodes the receptor for macrophage colony-stimulating factor (CSF-1). The gene is expressed selectively in the macrophage and trophoblast cell lineages. Previous studies have indicated that sequences in intron 2 control transcript elongation in tissue-specific and regulated expression of c-fms. In humans, an alternative promoter was implicated in expression of the gene in trophoblasts. We show that in mice, c-fms transcripts in trophoblasts initiate from multiple points within the 2-kilobase (kb) region flanking the first coding exon. A reporter gene construct containing 3.5 kb of 5' flanking sequence and the downstream intron 2 directed expression of enhanced green fluorescent protein (EGFP)…
Citation impact
- FWCI
- 6.53
- Percentile
- 100%
- References
- 72
Authors
9- RTR. Tedjo Sasmono
Albert Einstein College of Medicine, The University of Queensland, Yeshiva University, Canberra Hospital, ACT Government, The Ohio State University
- DODelvac Oceandy
Albert Einstein College of Medicine, The University of Queensland, Yeshiva University, Canberra Hospital, ACT Government, The Ohio State University
- JWJeffrey W. Pollard
Albert Einstein College of Medicine, The University of Queensland, Yeshiva University, Canberra Hospital, ACT Government, The Ohio State University
- WTWei Tong
Albert Einstein College of Medicine, The University of Queensland, Yeshiva University, Canberra Hospital, ACT Government, The Ohio State University
- PPPaul Pavli
Albert Einstein College of Medicine, The University of Queensland, Yeshiva University, Canberra Hospital, ACT Government, The Ohio State University
Topics & keywords
- Biology
- Molecular biology
- Transgene
- Intron
- Mononuclear phagocyte system
- Macrophage colony-stimulating factor
- Green fluorescent protein
- Enhancer