Development of High-Density Genetic Maps for Barley and Wheat Using a Novel Two-Enzyme Genotyping-by-Sequencing Approach

Advancements in next-generation sequencing technology have enabled whole genome re-sequencing in many species providing unprecedented discovery and characterization of molecular polymorphisms. There are limitations, however, to next-generation sequencing approaches for species with large complex genomes such as barley and wheat. Genotyping-by-sequencing (GBS) has been developed as a tool for association studies and genomics-assisted breeding in a range of species including those with complex genomes. GBS uses restriction enzymes for targeted complexity reduction followed by multiplex sequencing to produce high-quality polymorphism data at a relatively low per sample cost. Here we present a GBS approach for…

• UD
  U.S. Department of Agriculture

  Award: 2011-68002-30029
• BA
  Bill and Melinda Gates Foundation
• UO
  University of Missouri
• NI
  National Institute of Food and Agriculture

  Award: 2011-68002-30029
• AR
  Agricultural Research Service

  Award: 2011-68002-30029