Direct Evidence of Mesenchymal Stem Cell Tropism for Tumor and Wounding Microenvironments Using In Vivo Bioluminescent Imaging
The University of Texas MD Anderson Cancer Center
Abstract
Multipotent mesenchymal stromal/stem cells (MSC) have shown potential clinical utility. However, previous assessments of MSC behavior in recipients have relied on visual detection in host tissue following sacrifice, failing to monitor in vivo MSC dispersion in a single animal and limiting the number of variables that can be observed concurrently. In this study, we used noninvasive, in vivo bioluminescent imaging to determine conditions under which MSC selectively engraft in sites of inflammation. MSC modified to express firefly luciferase (ffLuc-MSC) were injected into healthy mice or mice bearing inflammatory insults, and MSC localization was followed with bioluminescent imaging. The inflammatory insults…
Citation impact
- FWCI
- 16.25
- Percentile
- 100%
- References
- 57
Authors
10- SKShannon Kidd
The University of Texas MD Anderson Cancer Center
- ELErika L. Spaeth
The University of Texas MD Anderson Cancer Center
- JLJennifer L. Dembinski
The University of Texas MD Anderson Cancer Center
- MDMartin Dietrich
The University of Texas MD Anderson Cancer Center
- KWKeri Watson
The University of Texas MD Anderson Cancer Center
Topics & keywords
- Mesenchymal stem cell
- Biology
- Bioluminescence imaging
- In vivo
- Tropism
- Pathology
- Luciferase
- Cancer research