Oxidative Stress Is Inherent in Prostate Cancer Cells and Is Required for Aggressive Phenotype

Reactive oxygen species (ROS) and the coupled oxidative stress have been associated with tumor formation. Several studies suggested that ROS can act as secondary messengers and control various signaling cascades. In the present studies, we characterized the oxidative stress status in three different prostate cancer cells (PC3, DU145, and LNCaP) exhibiting various degree of aggressiveness and normal prostate cells in culture (WPMY1, RWPE1, and primary cultures of normal epithelial cells). We observed increased ROS generation in cancer cells compared with normal cells, and that extramitochondrial source of ROS generator, NAD(P)H oxidase (Nox) systems, are associated with the ROS generation and are critical for…

• NI
  National Institutes of Health
• CC
  Cancer Center, University of Colorado
• NC
  National Cancer Institute
• UO
  University of Colorado School of Medicine, Anschutz Medical Campus