CUPSAT: prediction of protein stability upon point mutations

CUPSAT (Cologne University Protein Stability Analysis Tool) is a web tool to analyse and predict protein stability changes upon point mutations (single amino acid mutations). This program uses structural environment specific atom potentials and torsion angle potentials to predict DeltaDeltaG, the difference in free energy of unfolding between wild-type and mutant proteins. It requires the protein structure in Protein Data Bank format and the location of the residue to be mutated. The output consists information about mutation site, its structural features (solvent accessibility, secondary structure and torsion angles), and comprehensive information about changes in protein stability for 19 possible…

• IM
  International Max Planck Research School for Environmental, Cellular and Molecular Microbiology
• BF
  Bundesministerium für Bildung und Forschung
• IM
  International Max Planck Research School for Advanced Methods in Process and Systems Engineering