PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux
Charles University · Purdue University West Lafayette · +5 more institutions
Abstract
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This…
Citation impact
- FWCI
- 82.37
- Percentile
- 100%
- References
- 23
Authors
22- JPJan Petrášek
Charles University, Purdue University West Lafayette, Institute of Plant Biology, Bernstein Center for Computational Neuroscience Tübingen, Czech Academy of Sciences, Institute of Experimental Botany, University of Tübingen
- JMJozef Mravec
Charles University, Purdue University West Lafayette, Institute of Plant Biology, Bernstein Center for Computational Neuroscience Tübingen, Czech Academy of Sciences, Institute of Experimental Botany, University of Tübingen
- RBRodolphe Bouchard
Charles University, Purdue University West Lafayette, Institute of Plant Biology, Bernstein Center for Computational Neuroscience Tübingen, Czech Academy of Sciences, Institute of Experimental Botany, University of Tübingen
- JJJoshua J. Blakeslee
Charles University, Purdue University West Lafayette, Institute of Plant Biology, Bernstein Center for Computational Neuroscience Tübingen, Czech Academy of Sciences, Institute of Experimental Botany, University of Tübingen
- MAMelinda Abas
Charles University, Purdue University West Lafayette, Institute of Plant Biology, Bernstein Center for Computational Neuroscience Tübingen, Czech Academy of Sciences, Institute of Experimental Botany, University of Tübingen
Topics & keywords
- Auxin
- Efflux
- Cell biology
- Limiting
- Function (biology)
- Arabidopsis
- Biology
- Polar auxin transport
- Good health and well-being