Metagenome, metatranscriptome and single-cell sequencing reveal microbial response to Deepwater Horizon oil spill
Florida State University · Lawrence Berkeley National Laboratory · +6 more institutions
Abstract
The Deepwater Horizon oil spill in the Gulf of Mexico resulted in a deep-sea hydrocarbon plume that caused a shift in the indigenous microbial community composition with unknown ecological consequences. Early in the spill history, a bloom of uncultured, thus uncharacterized, members of the Oceanospirillales was previously detected, but their role in oil disposition was unknown. Here our aim was to determine the functional role of the Oceanospirillales and other active members of the indigenous microbial community using deep sequencing of community DNA and RNA, as well as single-cell genomics. Shotgun metagenomic and metatranscriptomic sequencing revealed that genes for motility, chemotaxis and aliphatic…
Citation impact
- FWCI
- 44.91
- Percentile
- 100%
- References
- 45
Authors
18- OUOlivia U. MasonCorresponding
Florida State University, Lawrence Berkeley National Laboratory
- TCTerry C. Hazen
Lawrence Berkeley National Laboratory, University of Tennessee at Knoxville
- SBSharon Borglin
Lawrence Berkeley National Laboratory
- PCPatrick Chain
Los Alamos National Laboratory, Joint Genome Institute
- EAEric A. Dubinsky
Lawrence Berkeley National Laboratory
Topics & keywords
- Metagenomics
- Biology
- Gene
- Genetics
- Life below water
Funding
- UDU.S. Department of EnergyAwards: -AC02-05CH11231, 05CH11231, AC02-05CH11231, DE-AC02, DE-AC02-05CH11231, DE-AC02-
- EBEnergy Biosciences InstituteAward: DE-AC02-05CH11231
- JGJoint Genome InstituteAwards: DE-AC02-05CH11231, AC02-05CH11231
- CUCurtin University of Technology
- OOOffice of ScienceAwards: AC02-05CH11231, -AC02-05CH11231, DE-AC02
- BEBasic Energy SciencesAwards: DE-AC02, AC02-05CH11231, DE-AC02-05CH11231, -AC02-05CH11231
- LBLawrence Berkeley National LaboratoryAwards: DE-AC02-05CH11231, 05CH11231, AC02-05CH11231