Comprehensive Characterization of Genes Required for Protein Folding in the Endoplasmic Reticulum
QB3 · Howard Hughes Medical Institute · +2 more institutions
Abstract
Protein folding in the endoplasmic reticulum is a complex process whose malfunction is implicated in disease and aging. By using the cell's endogenous sensor (the unfolded protein response), we identified several hundred yeast genes with roles in endoplasmic reticulum folding and systematically characterized their functional interdependencies by measuring unfolded protein response levels in double mutants. This strategy revealed multiple conserved factors critical for endoplasmic reticulum folding, including an intimate dependence on the later secretory pathway, a previously uncharacterized six-protein transmembrane complex, and a co-chaperone complex that delivers tail-anchored proteins to their membrane…
Citation impact
- FWCI
- 21.85
- Percentile
- 100%
- References
- 37
Authors
11- MCMartin C. Jonikas
QB3, Howard Hughes Medical Institute, University of California, San Francisco, University of Manchester
- SRSean R. Collins
QB3, Howard Hughes Medical Institute, University of California, San Francisco, University of Manchester
- VDVladimir DenicCorresponding
QB3, Howard Hughes Medical Institute, University of California, San Francisco, University of Manchester
- EOEugene Oh
QB3, Howard Hughes Medical Institute, University of California, San Francisco, University of Manchester
- EMErin M. Quan
QB3, Howard Hughes Medical Institute, University of California, San Francisco, University of Manchester
Topics & keywords
- Endoplasmic reticulum
- STIM1
- Cell biology
- Chaperone (clinical)
- Protein folding
- Biology
- Secretory pathway
- Secretory protein