Structural and Functional Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells
California Institute for Regenerative Medicine · University of Washington
Abstract
Despite preclinical studies demonstrating the functional benefit of transplanting human pluripotent stem cell-derived cardiomyocytes (PSC-CMs) into damaged myocardium, the ability of these immature cells to adopt a more adult-like cardiomyocyte (CM) phenotype remains uncertain. To address this issue, we tested the hypothesis that prolonged in vitro culture of human embryonic stem cell (hESC)- and human induced pluripotent stem cell (hiPSC)-derived CMs would result in the maturation of their structural and contractile properties to a more adult-like phenotype. Compared to their early-stage counterparts (PSC-CMs after 20-40 days of in vitro differentiation and culture), late-stage hESC-CMs and hiPSC-CMs (80-120…
Citation impact
- FWCI
- 24.38
- Percentile
- 100%
- References
- 52
Authors
4- SDScott D. Lundy
California Institute for Regenerative Medicine, University of Washington
- WZWei-Zhong Zhu
California Institute for Regenerative Medicine, University of Washington
- MRMichael RegnierCorresponding
California Institute for Regenerative Medicine, University of Washington
- MAMichael A. LaflammeCorresponding
California Institute for Regenerative Medicine, University of Washington
Topics & keywords
- Biology
- Induced pluripotent stem cell
- Sarcomere
- Cell biology
- Myofibril
- Embryonic stem cell
- Stem cell
- Myocyte