reviewReports on Progress in PhysicsOct 29, 2015Closed access

A review of progress in single particle tracking: from methods to biophysical insights

CMCarlo ManzoMGM.F. Garcia Parajo

Institute of Photonic Sciences · Institució Catalana de Recerca i Estudis Avançats

PubMed
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Abstract

Optical microscopy has for centuries been a key tool to study living cells with minimum invasiveness. The advent of single molecule techniques over the past two decades has revolutionized the field of cell biology by providing a more quantitative picture of the complex and highly dynamic organization of living systems. Amongst these techniques, single particle tracking (SPT) has emerged as a powerful approach to study a variety of dynamic processes in life sciences. SPT provides access to single molecule behavior in the natural context of living cells, thereby allowing a complete statistical characterization of the system under study. In this review we describe the foundations of SPT together with novel…

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2

Topics & keywords

Topics
Keywords
  • Physics
  • Context (archaeology)
  • Ergodicity
  • Living systems
  • Nanotechnology
  • Tracking (education)
  • Living cell
  • Function (biology)
UN Sustainable Development Goals
  • Responsible consumption and production
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