Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration

Although Apc is well characterized as a tumor-suppressor gene in the intestine, the precise mechanism of this suppression remains to be defined. Using a novel inducible Ahcre transgenic line in conjunction with a loxP-flanked Apc allele we, show that loss of Apc acutely activates Wnt signaling through the nuclear accumulation of beta-catenin. Coincidentally, it perturbs differentiation, migration, proliferation, and apoptosis, such that Apc-deficient cells maintain a "crypt progenitor-like" phenotype. Critically, for the first time we confirm a series of Wnt target molecules in an in vivo setting and also identify a series of new candidate targets within the same setting.

• PG
  Parc Geneteg Cymru
• CR
  Cancer Research UK
• DF
  Directorate for Biological Sciences
• BA
  Biotechnology and Biological Sciences Research Council