Quantitative targeted absolute proteomics of human blood–brain barrier transporters and receptors
Tohoku University · Azabu University
Abstract
We have obtained, for the first time, a quantitative protein expression profile of membrane transporters and receptors in human brain microvessels, that is, the blood-brain barrier (BBB). Brain microvessels were isolated from brain cortexes of seven males (16-77 years old) and protein expression of 114 membrane proteins was determined by means of a liquid chromatography-tandem mass spectrometric quantification method using recently established in-silico peptide selection criteria. Among drug transporters, breast cancer resistance protein showed the most abundant protein expression (8.14 fmol/μg protein), and its expression level was 1.85-fold greater in humans than in mice. By contrast, the expression level of…
Citation impact
- FWCI
- 25.55
- Percentile
- 100%
- References
- 58
Authors
7Topics & keywords
- Transporter
- Organic anion-transporting polypeptide
- Organic anion transporter 1
- Receptor
- Abcg2
- Blood–brain barrier
- Biology
- Organic cation transport proteins
- Good health and well-being