Drusen proteome analysis: An approach to the etiology of age-related macular degeneration
Cleveland Clinic Lerner College of Medicine · Case Western Reserve University · +2 more institutions
Abstract
Drusen are extracellular deposits that accumulate below the retinal pigment epithelium on Bruch's membrane and are risk factors for developing age-related macular degeneration (AMD). The progression of AMD might be slowed or halted if the formation of drusen could be modulated. To work toward a molecular understanding of drusen formation, we have developed a method for isolating microgram quantities of drusen and Bruch's membrane for proteome analysis. Liquid chromatography tandem MS analyses of drusen preparations from 18 normal donors and five AMD donors identified 129 proteins. Immunocytochemical studies have thus far localized approximately 16% of these proteins in drusen. Tissue metalloproteinase…
Citation impact
- FWCI
- 35.62
- Percentile
- 100%
- References
- 40
Authors
12- JWJohn W. CrabbCorresponding
Cleveland Clinic Lerner College of Medicine, Case Western Reserve University
- MMMasaru Miyagi
Cleveland Clinic Lerner College of Medicine, The California Eye Institute, Case Western Reserve University
- XGXiaorong Gu
Cleveland Clinic Lerner College of Medicine, Case Western Reserve University
- KSK.G. Shadrach
Cleveland Clinic Lerner College of Medicine, Case Western Reserve University
- KAKaren A. West
Cleveland Clinic Lerner College of Medicine, Case Western Reserve University
Topics & keywords
- Drusen
- Macular degeneration
- Vitronectin
- Retinal
- Biology
- Biochemistry
- Chemistry
- Extracellular matrix