UCHIME improves sensitivity and speed of chimera detection
Broad Institute · University of Colorado Boulder · +1 more institution
Abstract
MOTIVATION: Chimeric DNA sequences often form during polymerase chain reaction amplification, especially when sequencing single regions (e.g. 16S rRNA or fungal Internal Transcribed Spacer) to assess diversity or compare populations. Undetected chimeras may be misinterpreted as novel species, causing inflated estimates of diversity and spurious inferences of differences between populations. Detection and removal of chimeras is therefore of critical importance in such experiments. RESULTS: We describe UCHIME, a new program that detects chimeric sequences with two or more segments. UCHIME either uses a database of chimera-free sequences or detects chimeras de novo by exploiting abundance data. UCHIME has better…
Citation impact
- FWCI
- 294.18
- Percentile
- 100%
- References
- 21
Authors
5- RCR. C. EdgarCorresponding
Broad Institute, University of Colorado Boulder, University of Glasgow
- BJBrian J. Haas
Broad Institute, University of Colorado Boulder, University of Glasgow
- JCJosé C. Clemente
Broad Institute, University of Colorado Boulder, University of Glasgow
- CQChristopher Quince
Broad Institute, University of Colorado Boulder, University of Glasgow
- RKRob Knight
Broad Institute, University of Colorado Boulder, University of Glasgow
Topics & keywords
- Chimera (genetics)
- Computational biology
- Biology
- Spurious relationship
- Polymerase chain reaction
- Genetics
- Computer science
- Gene
- Life in Land