Epidermal Growth Factor Receptor in Non–Small-Cell Lung Carcinomas: Correlation Between Gene Copy Number and Protein Expression and Impact on Prognosis
University of Colorado Cancer Center · University of Colorado Denver
Abstract
Gene copy number and protein status of EGFR were investigated in microarrayed tumors from 183 NSCLC patients, including squamous cell carcinoma (SCC; 89 patients) and non-SCC (94 patients) histologies. Protein expression was assessed by immunohistochemistry on a scale from 0 to 400 (percentage of positive cells x staining intensity). Gene and chromosome 7 copy numbers were identified by fluorescent in situ hybridization (FISH).
EGFR protein overexpression was observed in 62% of the NSCLC (25% scored 201 to 300; 37% scored 301 to 400), more frequently in SCC than non-SCC (82% v 44%; P
Citation impact
- FWCI
- 49.22
- Percentile
- 100%
- References
- 35
Authors
9- FRFred R. HirschCorresponding
University of Colorado Cancer Center, University of Colorado Denver
- MVMarileila Varella‐Garcia
University of Colorado Cancer Center, University of Colorado Denver
- PAPaul A. Bunn
University of Colorado Cancer Center, University of Colorado Denver
- MVMichael V. Di Maria
University of Colorado Cancer Center, University of Colorado Denver
- RVRobert Veve
University of Colorado Cancer Center, University of Colorado Denver
Topics & keywords
- Polysomy
- Epidermal growth factor receptor
- Copy-number variation
- Fluorescence in situ hybridization
- Gene duplication
- Cancer research
- Immunohistochemistry
- Gefitinib