articleCancer ResearchJun 1, 2008GREEN OA

Identification and Characterization of Ovarian Cancer-Initiating Cells from Primary Human Tumors

Shanghai Jiao Tong University · Renji Hospital · +9 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

The objective of this study was to identify and characterize a self-renewing subpopulation of human ovarian tumor cells (ovarian cancer-initiating cells, OCICs) fully capable of serial propagation of their original tumor phenotype in animals. Ovarian serous adenocarcinomas were disaggregated and subjected to growth conditions selective for self-renewing, nonadherent spheroids previously shown to derive from tissue stem cells. To affirm the existence of OCICs, xenoengraftment of as few as 100 dissociated spheroid cells allowed full recapitulation of the original tumor (grade 2/grade 3 serous adenocarcinoma), whereas >10(5) unselected cells remained nontumorigenic. Stemness properties of OCICs (under stem…

No related works found for this paper.

Funding