Recombinant protein expression in Escherichia coli: advances and challenges
National University of Rosario · Instituto de Biología Molecular y Celular de Rosario · +1 more institution
Abstract
Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
Citation impact
- FWCI
- 55.77
- Percentile
- 100%
- References
- 238
Authors
2- GLGermán L. RosanoCorresponding
National University of Rosario, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas
- EAEduardo A. Ceccarelli
Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Biología Molecular y Celular de Rosario, National University of Rosario
Topics & keywords
- Escherichia coli
- Recombinant DNA
- Heterologous
- Plasmid
- Biology
- Heterologous expression
- Protein expression
- Computational biology