Multiple knockout mouse models reveal lincRNAs are required for life and brain development
Broad Institute · Harvard University · +4 more institutions
Abstract
Many studies are uncovering functional roles for long noncoding RNAs (lncRNAs), yet few have been tested for in vivo relevance through genetic ablation in animal models. To investigate the functional relevance of lncRNAs in various physiological conditions, we have developed a collection of 18 lncRNA knockout strains in which the locus is maintained transcriptionally active. Initial characterization revealed peri- and postnatal lethal phenotypes in three mutant strains (Fendrr, Peril, and Mdgt), the latter two exhibiting incomplete penetrance and growth defects in survivors. We also report growth defects for two additional mutant strains (linc-Brn1b and linc-Pint). Further analysis revealed defects in lung,…
Citation impact
- FWCI
- 26.90
- Percentile
- 100%
- References
- 78
Authors
25- MSMartin SauvageauCorresponding
Broad Institute, Harvard University
- LALoyal A. Goff
Broad Institute, Harvard University, Massachusetts Institute of Technology
- SLSimona Lodato
Broad Institute, Harvard University
- BBBoyan Bonev
Broad Institute, Harvard University
- AFAbigail F. Groff
Broad Institute, Harvard University
Topics & keywords
- Biology
- Penetrance
- Phenotype
- Knockout mouse
- Mutant
- Genetics
- Neocortex
- CRISPR