Identification and microbial production of a terpene-based advanced biofuel
Lawrence Berkeley National Laboratory · Joint BioEnergy Institute · +1 more institution
Abstract
Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l−1. We produce bisabolene in Saccharomyces cerevisiae (>900 mg l−1), a…
Citation impact
- FWCI
- 16.89
- Percentile
- 100%
- References
- 25
Authors
6- PPPamela Peralta‐YahyaCorresponding
- MOMario Ouellet
Lawrence Berkeley National Laboratory, Joint BioEnergy Institute
- RCRossana Chan
Joint BioEnergy Institute, Lawrence Berkeley National Laboratory
- AMAindrila Mukhopadhyay
Joint BioEnergy Institute, Lawrence Berkeley National Laboratory
- JDJay D. Keasling
University of California, Berkeley, Joint BioEnergy Institute, Lawrence Berkeley National Laboratory
Topics & keywords
- Biofuel
- Metabolic engineering
- Terpene
- Chemistry
- Lignocellulosic biomass
- Biomass (ecology)
- Diesel fuel
- Biochemical engineering
Funding
- UDU.S. Department of EnergyAwards: -AC02-05CH11231, 05CH11231, AC02-05CH11231, DE-AC02, DE-AC02-05CH11231, DE-AC02-
- WSWashington State University
- BABiological and Environmental ResearchAwards: 05CH11231, DE-AC02-05CH11231, AC02-05CH11231
- NRNational Renewable Energy LaboratoryAward: DE-AC02-05CH11231
- LBLawrence Berkeley National LaboratoryAwards: DE-AC02-05CH11231, 05CH11231, AC02-05CH11231