articleGeneticsMay 25, 2013BRONZE OA

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

SJScott J. GratzAMAlexander M CummingsJNJennifer NguyenDCDanielle C. HammLKLaura K. Donohue

University of Wisconsin–Madison

PubMed
Indexed incrossrefpubmed

Abstract

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

Citation impact

1,059
total citations
FWCI
52.19
Percentile
100%
References
33
Citations per year

Authors

8

Topics & keywords

Topics
Keywords
  • Biology
  • CRISPR
  • Nuclease
  • Genetics
  • Cas9
  • Genome engineering
  • Genome editing
  • Genome
No related works found for this paper.

Funding