Particokinetics In Vitro: Dosimetry Considerations for In Vitro Nanoparticle Toxicity Assessments

The rapid growth in the use of in vitro methods for nanoparticle toxicity assessment has proceeded with limited consideration of the unique kinetics of these materials in solution. Particles in general and nanoparticles specifically, diffuse, settle, and agglomerate in cell culture media as a function of systemic and particle properties: media density and viscosity and particle size, shape, charge and density, for example. Cellular dose then is also a function of these factors as they determine the rate of transport of nanoparticles to cells in culture. Here we develop and apply the principles of dosimetry in vitro and outline an approach for simulation of nanoparticle particokinetics in cell culture systems.…

• UD
  U.S. Department of Energy

  Awards: 76RL01830, AC05-76RL01830, Contract DE-AC05-76RL01830, DE-AC05-76RL01830, DE-AC05
• B
  Battelle

  Awards: AC05-76RL01830, DE-AC05, DE-AC05-76RL01830
• LD
  Laboratory Directed Research and Development

  Awards: 76RL01830, DE-AC05-76RL01830
• PN
  Pacific Northwest National Laboratory

  Awards: DE-AC05-76RL01830, 76RL01830