Identification of novel NRF2-regulated genes by ChIP-Seq: influence on retinoid X receptor alpha
National Institute of Environmental Health Sciences · National Institutes of Health · +1 more institution
Abstract
Cellular oxidative and electrophilic stress triggers a protective response in mammals regulated by NRF2 (nuclear factor (erythroid-derived) 2-like; NFE2L2) binding to deoxyribonucleic acidregulatory sequences near stress-responsive genes. Studies using Nrf2-deficient mice suggest that hundreds of genes may be regulated by NRF2. To identify human NRF2-regulated genes, we conducted chromatin immunoprecipitation (ChIP)-sequencing experiments in lymphoid cells treated with the dietary isothiocyanate, sulforaphane (SFN) and carried out follow-up biological experiments on candidates. We found 242 high confidence, NRF2-bound genomic regions and 96% of these regions contained NRF2regulatory sequence motifs. The…
Citation impact
- FWCI
- 14.51
- Percentile
- 100%
- References
- 114
Authors
10- BNB. N. ChorleyCorresponding
National Institute of Environmental Health Sciences
- MRMatthew R. Campbell
National Institutes of Health, National Institute of Environmental Health Sciences
- XWXuting Wang
National Institutes of Health, National Institute of Environmental Health Sciences
- MKMehmet Karaca
National Institutes of Health, National Institute of Environmental Health Sciences
- DSDeepa Sambandan
National Institutes of Health, National Institute of Environmental Health Sciences
Topics & keywords
- Biology
- Retinoid X receptor
- Gene knockdown
- Nuclear receptor
- Chromatin immunoprecipitation
- Retinoid X receptor alpha
- Transcription factor
- Adipogenesis