Chemical derivatization and mass spectral libraries in metabolic profiling by GC/MS and LC/MS/MS
Royal Holloway University of London · University of London · +1 more institution
Abstract
An overview is presented of gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS), the two major hyphenated techniques employed in metabolic profiling that complement direct 'fingerprinting' methods such as atmospheric pressure ionization (API) quadrupole time-of-flight MS, API Fourier transform MS, and NMR. In GC/MS, the analytes are normally derivatized prior to analysis in order to reduce their polarity and facilitate chromatographic separation. The electron ionization mass spectra obtained are reproducible and suitable for library matching, mass spectral collections being readily available. In LC/MS, derivatization and library matching are at an early stage of…
Citation impact
- FWCI
- 7.35
- Percentile
- 100%
- References
- 155
Authors
6- JMJohn M. HalketCorresponding
Royal Holloway University of London
- DWDaniel Waterman
University of London, Bioanalytica (Switzerland)
- APA. Przyborowska
Royal Holloway University of London
- RPRaj Patel
University of London, Royal Holloway University of London, Bioanalytica (Switzerland)
- PDPaul D. Fraser
University of London, Bioanalytica (Switzerland)
Topics & keywords
- Derivatization
- Chemistry
- Mass spectrometry
- Chromatography
- Atmospheric-pressure chemical ionization
- Formic acid
- Gas chromatography–mass spectrometry
- Chemical ionization