Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
University of California, San Francisco · Center for Integrated Protein Science Munich · +3 more institutions
Abstract
Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations…
Citation impact
- FWCI
- 87.82
- Percentile
- 100%
- References
- 29
Authors
12- LSLothar SchermellehCorresponding
University of California, San Francisco, Center for Integrated Protein Science Munich, Max Delbrück Center, University of Florida, Ludwig-Maximilians-Universität München
- PMPeter M. CarltonCorresponding
University of California, San Francisco, Center for Integrated Protein Science Munich, Max Delbrück Center, University of Florida, Ludwig-Maximilians-Universität München
- SHSebastian Haase
University of California, San Francisco, Center for Integrated Protein Science Munich, Max Delbrück Center, University of Florida, Ludwig-Maximilians-Universität München
- LSLin Shao
University of California, San Francisco, Center for Integrated Protein Science Munich, Max Delbrück Center, University of Florida, Ludwig-Maximilians-Universität München
- LWLukman Winoto
University of California, San Francisco, Center for Integrated Protein Science Munich, Max Delbrück Center, University of Florida, Ludwig-Maximilians-Universität München
Topics & keywords
- Nuclear lamina
- Microscopy
- Lamin
- Nuclear pore
- Chromatin
- Fluorescence microscope
- Super-resolution microscopy
- Nucleus