Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins and are of great medical interest; serving as novel biomarkers and potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield and high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) and differential ultracentrifugation (UC). A significantly higher EV yield resulted from UF-LC as compared to UC, without affecting vesicle protein composition. Importantly, we provide novel evidence that, in contrast to…

• AF
  Agency for Science, Technology and Research
• ET
  Eesti Teadusfondi
• C
  Cancerfonden
• SS
  Svenska Sällskapet för Medicinsk Forskning
• H
  Hjärt-Lungfonden
• KI
  Karolinska Institutet
• V
  Vetenskapsrådet
• B
  BIRAX