reviewClinical Microbiology ReviewsJan 1, 2015BRONZE OA

The Rebirth of Culture in Microbiology through the Example of Culturomics To Study Human Gut Microbiota

JLJean‐Christophe LagierPHPerrine HugonSKS. KhelaifiaPFPierre‐Edouard FournierBLBernard La Scola

Centre National de la Recherche Scientifique · Inserm · +3 more institutions

PubMed
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Abstract

Bacterial culture was the first method used to describe the human microbiota, but this method is considered outdated by many researchers. Metagenomics studies have since been applied to clinical microbiology; however, a "dark matter" of prokaryotes, which corresponds to a hole in our knowledge and includes minority bacterial populations, is not elucidated by these studies. By replicating the natural environment, environmental microbiologists were the first to reduce the "great plate count anomaly," which corresponds to the difference between microscopic and culture counts. The revolution in bacterial identification also allowed rapid progress. 16S rRNA bacterial identification allowed the accurate…

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740
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Authors

6

Topics & keywords

Topics
Keywords
  • Metagenomics
  • Identification (biology)
  • Pyrosequencing
  • Prokaryote
  • Biology
  • Computational biology
  • Repertoire
  • Human microbiome
UN Sustainable Development Goals
  • Life in Land
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