Isothermal Amplification of Nucleic Acids

Zhao, Yongxi; Chen, Feng; Li, Qian; Wang, Lihua; Fan, Chunhai

doi:10.1021/acs.chemrev.5b00428

reviewChemical ReviewsNov 9, 2015Closed access

Isothermal Amplification of Nucleic Acids

YZYongxi Zhao FCFeng Chen QLQian Li LWLihua Wang CFChunhai Fan

Xi'an Jiaotong University · Shanghai Institute of Applied Physics · +1 more institution

PubMed

Indexed incrossrefpubmed

Abstract

Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently accumulates nucleic acid sequences at constant temperature. Since the early 1990s, various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR). These isothermal amplification methods have been used for biosensing targets such as DNA, RNA, cells, proteins, small molecules, and ions. The applications of these techniques for in situ or intracellular bioimaging and sequencing have been amply demonstrated. Amplicons produced by isothermal amplification methods have also been utilized to construct versatile nucleic acid nanomaterials for promising applications in…

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Topics & keywords

Topics

Keywords

Loop-mediated isothermal amplification
Nucleic acid
Chemistry
Amplicon
Nucleic acid quantitation
Rolling circle replication
Isothermal process
Microfluidics

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